2022年EPG廠家培訓(xùn)

時間：12.11-12.13，地點：墨爾本（澳大利亞）

當(dāng)?shù)刎?fù)責(zé)人：

Piotr Trebicki & Isabel Valanzuela（奧地利）

項目負(fù)責(zé)人：

Freddy Tjallingii（荷蘭），Elisa Garzo & Alberto Fereres（西班牙）

尊敬的同行：

什么是EPG（刺探電位圖）技術(shù)？

      將植物和昆蟲（大多是同翅目昆蟲）分別通過植物電極和昆蟲電極連接，構(gòu)成連通電路的一部分；植物電極插入植物生長的土壤中，而昆蟲電極是通過銀膠將一根非常細(xì)的金線粘在昆蟲的背板部位而形成。當(dāng)昆蟲借助刺吸式口器取食植物時，由于口器刺入植物組織的部位不同，以及昆蟲的活動（機(jī)體活動、吸食或流涎），導(dǎo)致連通電路的電勢差變化，即波形的波動。通常情況下，可以一次記錄持續(xù)8小時的8個重復(fù)實驗（可能還有更多的實驗配置方法）

     EPG常用于研究植物與昆蟲之間的相互作用，包括植物抗性、宿主植物識別、植物病原體傳播、植物防御的電生理方面等。

課程內(nèi)容：包括講座（上午）和實操（下午），在法拉第籠中使用、昆蟲和植物的布線安裝、監(jiān)測記錄（數(shù)據(jù)采集）、信號分析、數(shù)據(jù)處理和統(tǒng)計分析方向。

參會人數(shù)有限：由于設(shè)備和設(shè)施的限制，參會者最多16人，若有更多人注冊，將會使用候補(bǔ)名單。

會議費：

500澳元（約人民幣2500元）

參會方式：回復(fù)郵件如下或直接回復(fù)當(dāng)?shù)刎?fù)責(zé)人

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EPG培訓(xùn)課程日常安排：epgsystems.eu

附英文通知：

HPIS 2022 satellite EPG training course

December 11-13, Melbourne, Australia

Local Organizers:

Piotr Trebicki & Isabel Valanzuela (Australia)

Program organizers:

Freddy Tjallingii (Netherlands), Elisa Garzo & Alberto Fereres (Spain)

Dear colleague,

What is the EPG (Electrical Penetration Graphs) technique?

A plant and a (mostly) Homopteran insect are made part of an electrical circuit by using a soil electrode for the plant and gluing a very thin gold wire to insect’s dorsum. Upon plant penetration by the insect, electrically caused waveforms indicate tissue positions of the stylet tips and insect activities (mechanical movements, ingestion, or salivation). Typically, 8 replicates can be recorded for periods up to 6-8h (more experimental configurations are possible).

EPG recording is commonly used to study plant-insect interactions as involved plant resistance, host plant recognition, plant pathogen transmission,  electrophysiological aspects of plant defenses, etc.

Course program: see attachment. This includes lectures (morning sessions) of and practical training (afternoon sessions) in Faraday case use, wiring and mounting insects and plants, monitored recording (data acquisition), signal analysis, data processing and directions for statistical analysis.

Number of participants limit. Because of equipment and facilities limitations there will be a maximum of 16 participants. When more people sign up a waiting list will be used.

Fees. 

Participants pay a fee of AUS $500

Registration:

Please reply to this mail or to one of the local organizers

Best regards,

Freddy Tjallingii